The use of PCR in the analysis of an inherited condition was
first demonstrated in the detection of a common -globin
mutation in 1985. Since then, PCR has become an
indispensable technique for all laboratories involved in DNA
analysis. The technique requires the DNA sequence in the gene
or region of interest to have been elucidated. This limitation is
becoming increasingly less problematic with the pending
completion of the entire human DNA sequence.
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