Heteroduplexes are double-stranded DNA molecules that are
formed from two complementary strands that are imperfectly
matched. If a mutation is present in one copy of a gene being
amplified using PCR, heteroduplexes will be formed from
the hybridisation of the normal and the mutant PCR product.
As in SSCP analysis described above, these structures will have
altered mobility when analysed through non-denaturing
polyacrylamide gels, and are seen as band shifts when
compared to perfectly matched PCR products (or
homoduplexes).
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment