Agarose gel electrophoresis

Agarose gel electrophoresis is of sufficient resolution to
allow the rapid detection of the deletion of whole exons, which
is often seen in affected male DMD patients. In this approach,
a number of exons of the DMD gene are simultaneously
amplified in a “multiplex” PCR approach. Samples with exon
deletions are readily detected by the absence of specific bands
when analysed by agarose gel electrophoresis.