Genomic DNA is usually isolated from EDTA-anticoagulated
whole blood, often using an automated method. In addition,
DNA can also be readily isolated from fresh or frozen tissue
samples, chorionic villus biopsies, cultured amniocytes and
lymphoblastoid cell lines. Smaller quantities of DNA can be
recovered from buccal mouthwash samples and fixed
embedded tissues, although the recovery is considerably less
reliable. The increased use of the polymerase chain reaction
(PCR) means that for a small proportion of analyses, blood
volumes of 1 ml are adequate. In many instances however,
larger volumes of blood are still required because numerous
tests are required when analysing large or multiple genes and
not all tests use PCR based methods of analysis.
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